NONSPECIFIC ENVIRONMENTAL STRESSES AND THEIR EFFECTS ON EMBRYONIC DEVELOPMENT OF DROSOPHILA MELANOGASTER
Dihae G.Yook, Monica Lacy.
Vanderbilt University, Nashville, TN.
Nonspecific stresses such as heat stress, ultraviolet radiation, and hypoxic conditions applied to Drosophila melanogaster embryos at the onset of gastrulation causes defects later in embryonic development. In heat stress conditions, potential premature cellular death can be a proponent to defects during embryonic development of Drosophila. It is hypothesized that an applied heat shock will result in premature apoptotic events that can lead to holes in the amnioserosa. Heat stress was applied by placing gastrulating Drosophila embryos in a 38 °C water bath for 30 minutes. Ultra-violet radiation stress and hypoxic stress were 2 additional stresses applied to Drosophila embryos. Ultra-violet radiation was applied at 1.7 nJ for 2 minutes, and hypoxic stress was applied for 30 minutes. Embryos were allowed to recover post shock. In conclusion, there were several defects, but the most prevalent defects were holes in the amnioserosa, failure in germband retraction, failure in germband extension, and head defects in the Drosophila melanogaster embryo in all stress-induced conditions.