TARGETING GROWTH AND INVASIVENESS IN CANCER CELLS
Stephanie Soriano, Nitika Parmar.
California State University Channel Islands, Camarillo, CA.
Invasiveness is a characteristic feature of malignancy in cancer and can greatly reduce the 5-year survival rate of patients affected. This study aimed to determine the impact of 5 proposed anti-cancer agents (berberine chloride, azathioprine, gossypol, miltefosine, and etoposide) on 3 female cancer cell lines: MES-SA (uterine), C33A (cervical), and SKBR (breast). Invasiveness was tested using the CultreCoat® 96 Well Medium BME Cell Invasion Assay. The cancer cell lines were grown to 80 - 90% confluency and then treated with the proposed anti-cancer agents at 2 µM for 24 hours. Cells were harvested and subsequently seeded in the invasion chamber for 24 hours. Migration of the cells reflecting invasiveness was monitored via a fluorimetric assay and quantified. Untreated cells served as controls. Invasiveness was influenced by the type of reagent and cell line. Etoposide and berberine chloride greatly reduced invasiveness in a majority of the cell lines. A dose-dependent impact on invasiveness is currently being studied. Targeting invasiveness in these aggressive cell lines may lead to a potential direction.