INTERACTIONS OF STRESS AND 3-IODOTHYRONAMINE TREATMENT ON GENE EXPRESSION IN MCF-7 CELLS
Christopher Ponce, Shaikh Rahman.
Texas Tech University, Lubbock, TX.
The present study assessed the effects of 3-iodothyronamine (T1AM) treatment and cell-density mediated-oxidative stress on the expression of 3 different genes implicated in stress: hypoxia-inducible factor 1-alpha (HIF1α), pyruvate dehydrogenase alpha 1 (PDH1α), and lactate dehydrogenase alpha (LDHα). We hypothesized that both T1AM and cell-density mediated oxidative stress affect HIF1α, PDHα, and LDHα gene expression in MCF-7 cells. To test our hypothesis, non-invasive MCF-7 (ER+) breast cancer cells were cultured under standard culture conditions (5% CO2) and incubated at 37 °C. Cells were seeded at 50k, 100k, and 150k in Dulbeccos’s modified eagle’s medium (DMEM). The cells were then treated with either 50 mM T1AM or DMSO (control) for 24 hours. After that, cells were harvested for total RNA extraction and gene expression analyses. Cells in the plate those were seeded at 100k and treated with 50 mM T1AM showed moderate increase in HIF1α and LDHα gene expression. In conclusion, our data indicated that the cell-density mediated oxidative stress along with the T1AM treatment are responsible for moderate increase in H1F1α and LDHα gene expression in MCF-7 cells.