DEFINING THE ROLE OF THE NOVEL PROTEIN CG1674 IN ADULT MUSCLE DEVELOPMENT
Marilyn Cisneros, Richard Cripps.
The University of New Mexico, Albuquerque, NM.
In Drosophila, the study of muscle formation and structure can provide a better understanding of the factors that trigger muscle disease in humans. Our current goal is to determine if the protein CG1674 is a component of the sarcomere and if it is required for muscle structure and function. CG1674 was discovered through proteome sequencing of dissected flight muscles. This sequencing data suggests that this protein is a functional component of the flight muscles of Drosophila. We are currently generating a CG1674-GFP fusion protein to determine the localization of our protein within the muscle cell. In parallel, we are determining the requirement for CG1674 in normal muscle formation by expressing in the flight muscles an RNAi targeting the CG1674 transcript. When crossing UAS-CG1674 RNAi with the drivers Mef2Gal4 and 1151Gal4, the flies are flightless. Immunohistochemical staining of the muscles reveals defects in myofibril formation and structure indicating that the presence of the CG1674 protein is required for sarcomere assembly. In addition to creating our CG1674–GFP fusion construct, we are working on creating a knockout using the CRISPR method. The results will indicate the impact this protein has on flight muscles when it is absent. These studies identify a potentially novel muscle protein that is required for normal muscle assembly and function.